**INTERDISCIPLINARY RESEARCH**
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Membrane protein production and studies of structure-function relationships of membrane proteins in microbes, plants, humans and other organisms and in antimicrobial resistance, infectious and foodborne diseases investigated using chemical, biochemical, biophysical, molecular imaging and computational techniques - including nuclear magnetic resonance (NMR) spectroscopy - and the development of methods to achieve this.
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Publications
Ali M, Ali R, Khan IA, Ahmad I, Nawaz N, Patching SG. The Escherichia coli zinc exporter ZitB of the Cation Diffusion Facilitator (CDF) protein family: Properties, cloning, amplified expression, purification. viXra Preprints 2025: 2504.0050. https://vixra.org/abs/2504.0050
Patching SG. Ultra-High Field Nuclear Magnetic Resonance (NMR) Spectroscopy: Applications at 1 GHz and Beyond. International Journal of Current Science Research and Review 2025; 8(1): 336-351. DOI: https://doi.org/10.47191/ijcsrr/V8-i1-3
Nawaz N, Ahmad I, Patching SG, Hussain I. Tribulus terrestris fruit’s potential for synthesizing gold nanoparticles with remarkable biological prospects. Journal of Biological Research 2025. DOI: 10.4081/jbr.2025.12674
Ahmad I, Nawaz N, Afshar S, Saidijam M, Patching, SG. (2024). Roles of Nucleoside Transporters and the Nucleoside Analogue Radiotracer 3’-Deoxy-3’-[18F]Fluoro-L-Thymidine ([18F]FLT) in PET Imaging of Cancer Cells. In: Oluwagbemiga OF (Ed.) Medicine and Medical Research: New Perspectives Vol. 4, 103-137.
Patching SG. Structure and substrate recognition of the Escherichia coli transport protein NupG from the Nucleoside:H+ Symporter (NHS) family. International Journal of Current Science Research and Review 2024; 7(6): 3863-3868.
Ahmad I, Ali M, Ali R, Nawaz N, Patching SG. Multidrug Efflux Protein Families in Bacteria: ABC, RND, MFS, SMR, MATE, PACE, AbgT (An Update). viXra Preprint 2024. https://vixra.org/abs/2406.0087
Patching SG. Ultra-High Field Nuclear Magnetic Resonance (NMR) Spectroscopy: Applications at 1 GHz and Beyond. OSF Preprints 2024. DOI: 10.31219/osf.io/gte42
Patching SG. Structure and substrate recognition of the Escherichia coli transport protein NupG from the Nucleoside:H+ Symporter (NHS) family. OSF Preprints 2024. DOI: 10.31219/osf.io/ht9dg
Nawaz N, Patching SG. Polarising agents and spin tags used in DNP-ssNMR studies on biological samples: An update. Innovations in Biological Science (Vol. 2) 2024, pp. 123-164. DOI: 10.9734/bpi/ibs/v2/8303E
Nawaz N, Ali R, Ali M, Manfield IW, Taj MK, Mustafa MZ, Patching SG. Microscale Thermophoresis Analysis of Membrane Proteins. Chemical Science International Journal 2024; 33(2): 25-45. DOI: 10.9734/CSJI/2024/v33i2887.
Nawaz N, Patching SG. Polarising Agents and Spin Tags for Dynamic Nuclear Polarisation (DNP)-Enhanced Solid-State Nuclear Magnetic Resonance (ssNMR) Analysis of Biological Samples. Current Journal of Applied Science and Technology, 2023; 42(28): 11-38.
Nawaz N, Ali R, Ali M, Manfield IW, Taj MK, Mustafa MZ, Patching SG. Microscale Thermophoresis Analysis of Membrane Proteins. OSF Preprints 2024. DOI: 10.31219/osf.io/pe7ut
Nawaz N, Patching SG. Polarising Agents and Spin Tags for Dynamic Nuclear Polarisation (DNP)-Enhanced Solid-State Nuclear Magnetic Resonance (ssNMR) Analysis of Biological Samples. OSF Preprints 2023. DOI: 10.31219/osf.io/pyjuk
Sun L, Patching SG. Elevator mechanism of alternating access in the Escherichia coli concentrative nucleoside transporter NupC. Int J Adv Multidisc Res Stud 2023; 3(2): 888-910.
Patching SG. Structural and functional mechanisms of membrane proteins: Implications in health and disease (Editorial). Int J Mol Sci 2023.
Patching SG. Bacterial efflux proteins (Editorial). Int J Mol Sci 2023.
Ahmad I, Hassan KA, Henderson PJF, Patching SG. Cloning, amplified expression, functional characterisation and purification of a Pseudomonas putida NCS1 family transport protein. Int J Adv Multidiscip Res 2022; 9(12): 127-156. DOI: 10.22192/ijamr.2022.09.12.01
Basnet S, Marahatha R, Shrestha A, Bhattarai S, Katuwal S, Sharma KR, Marasini BP, Dahal SR, Basnyat RC, Patching SG, Parajuli N. In vitro and in silico studies for the identification of potent metabolites of some high-altitude medicinal plants from Nepal inhibiting SARS-CoV-2 spike protein. Molecules 2022; 27(24): 8957. DOI: 10.3390/molecules27248957
Patching SG. Spermidine binding to the Acetinobacter baumannii efflux protein AceI observed by near-UV synchrotron radiation circular dichroism spectroscopy. Radiation 2022; 2(2): 228-233. DOI: 10.3390/radiation2020016
Ahmad I, Ali M, Ali R, Nawaz N, Patching SG. Structure-based virtual screening and molecular docking of drugs against the SARS-CoV-2 spike protein-ACE2 receptor complex. Pak J Pharm Sci 2022, 35(6): 1531-1538. DOI: 10.36721/PJPS.2022.35.6.REG.1531-1538.1
Nadar S, Khan T, Patching SG, Omri A. Development of antibiofilm therapeutics strategies to overcome antimicrobial drug resistance. Microorganisms 2022; 10(2): 303. DOI: 10.3390/microorganisms10020303
Zia-Ud-Din, Aftab MN, Kakar NH, Mustafa MZ, Zafar A, Iqbal I, Ahmad I, Patching SG, Khan MA, Asmat TM, Shafee M, Rafeeq M. Comparative effects of Bacillus probiotic and antibiotic on the growth performance of indigenous Aseel chickens. Int J Agric Biol Sci 2022; 4: 37-45.
Ahmad I, Lee Y, Nawaz N, Elahi R, Khan IA, Mustafa MZ, Patching SG. Cloning, amplified expression and bioinformatics analysis of a putative Nucleobase Cation Symporter-1 (NCS-1) protein from Rhodococcus erythropolis. BioScientific Review 2021; 3(4). DOI: 10.32350//BSR.0304.05
Tayyeb M, Rashid N, Kakar NH, Taj MK, Abbasi BHA, Ahmad I, Patching SG, Kakar H, Mustafa MZ. Microbial and nutritional evaluation of fresh and wastewater cultivated cabbage in Quetta, Pakistan. Int J Environ Agric Res 2021; 7(5): 15-19.
Ahmad I, Khattak S, Ali R, Nawaz N, Ullah K, Khan SB, Ali M, Patching SG, Mustafa MZ. Prevalence and molecular characterization of multidrug-resistant Escherichia coli O157: H7 from dairy milk in the Peshawar region of Pakistan. J Food Safety 2021; 41(6): e12941. DOI: 10.1111/jfs.12941
Nawaz N, Ahmad I, Patching SG, Hussain I. Green synthesis of gold nanoparticles using Tribulus terrestris extract and antibacterial activity against Gram-negative bacteria. Biomed Lett 2021; 7(2): 80-89. DOI: 10.47262/BL/7.2.20210617
Patching SG. Bacterial multidrug efflux proteins in antimicrobial resistance (Editorial). Antibiotics 2021.
Ahmad I, Hassan KA, Henderson PJF, Patching SG. Cloning, amplified expression, functional characterisation and purification of a Pseudomonas putida NCS1 family transport protein. Preprint 2021.
Ahmad I, Hassan KA, Sharples DJ, Paulsen IT, Patching SG, Henderson PJF. Expression screening and purification of PACE family multidrug efflux proteins. Preprint 2021.
Rabail R, Saleem J, Tanveer Z, Patching SG, Khalid AR, Sultan MT, Manzoor MF, Karrar E, Inam-Ur-Raheem M, Shabbir MA, Aadil RM. Nutritional and lifestyle changes required for minimizing the recovery period in home quarantined COVID‐19 patients of Punjab, Pakistan. Food Sci Nutr 2021; 9(9): 5036-5039.
Patching SG, Martins M, Murakami S, Yan A, Yishino K, Law CJ, Nandanwar HS, Bay DC. Structure, function, and regulation of bacterial efflux proteins in metal homeostasis and antimicrobial resistance (Editorial). Front Mol Biosci 2021.
Ahmad I, Nawaz N, Dermani FK, Kohlan AK, Saidijam M, Patching SG. Bacterial multidrug efflux proteins: A major mechanism of antimicrobial resistance. Curr Drug Targets 2020 [Epub ahead of print] DOI: 10.2174/1389450119666180426103300.
Sun L, Xie H, Ingram JC, Hope RJ, Baldwin SA, Patching SG. Characterization of the Escherichia coli concentrative nucleoside transporter NupC using computational, biochemical and biophysical methods. Biochemistry 2020 [Epub ahead of print] DOI: 10.1021/acs.biochem.7b00172.
Tanveer Z, Ahmad I, Javed MS, Malik SA, Kakr NH, Ahmad M, Patching SG, Naeem M, Mustafa MZ. Screening for hepatitis b and c viral infections among pregnant women attending the Bolan Medical Complex Hospital and Sandeman Provincial Civil Hospital Quetta, Pakistan. Professional Med J 2020; 27(7): 1328-1334. DOI: 10.29309/TPMJ/2020.27.07.3129.
Ali R, Jiao Y, Wall PK, Patching SG, Ahmad I, Lutfulla G, dePamphilis CW. In silico identification and structure function analysis of a putative coclaurine N-methyltransferase from Aristolochia fimbriata. Comput Biol Chem 2020; 85: 107201.
Robajac D, Šunderić M, Gligorijević N, Nedić O, Tingey M, Schnell SJ, Li Y, Junod S, Yu W, Yang W, Roy NK, Beckstein O, Naughton F, Lu M, Lu KS-J, Lekshmi M, Wenzel N, Kumar SH, Varela MF, Turner RJ, Ahmad I, Ma P, Nawaz N, Sharples DJ, Henderson PJF, Patching SG. A Closer Look at Membrane Proteins. Independent Publishing Network, UK, 2020, pp. 1-350. ISBN: 978-1-83853-535-3.
Ahmad I, Ma P, Nawaz N, Sharples DJ, Henderson PJF, Patching SG. Cloning, amplified expression, functional characterisation and purification of Vibrio parahaemolyticus NCS1 cytosine transporter VPA1242. Chapter 8. In: Patching SG (Ed) A Closer Look at Membrane Proteins. Independent Publishing Network, UK, 2020, pp. 241-267. ISBN: 978-1-83853-535-3.
Jehan M, Bajwa MA, Tariq MM, Eyduran E, Rafeeq M, Mustafa MZ, Ahmad I, Patching SG, Waheed A, Ahmad J, Khan MA, Ahmad F, Fatih A, Ali I, Ahmed M, Amin S. Unique characteristics, biometric measurements, management and performance parameters of Balochi sheep. Int J Agric Biol Sci 2020; 4(5): 1-6.
Nawaz N, Ahmad I, Darwesh NM, Wahab A, ur Rahman S, Sajid A, Khan FA, Khan SB, Patching SG, Uddin K. Synthesis, characterization and antioxidant activity of Nickel(II) Schiff base complexes derived from 4-(dimethylamino)benzaldehyde. J Chem Soc Pak 2020; 42(2): 238-242.
Samad A, Abbas F, Tanveer Z, Ahmad Z, Ahmad I, Patching SG, Nawaz N, Asmat MT, Raziq A, Asasullah, Sheikh IS, Naeem M, Pokryshko O, Mustafa MZ. Prevalence of Salmonella spp. in chicken meat from Quetta retail outlets and typing through multiplex PCR. Rom Biotech Lett 2019; 24(2): 271-279.
Hassan KA, Naidu V, Edgerton JR, Mettrick KA, Liu Q, Fahmy L, Li L, Jackson SM, Ahmad I, Sharples D, Patching SG, Henderson PJF, Paulsen IT. Short-chain diamines are the physiological substrates of PACE family efflux pumps. Proc Natl Acad Sci U S A 2019; 116(36): 18015-18020.
Shafee M, Abbas F, Tanveer Z, Whitelaw A, Tow LA, Ashraf M, Ahmad I, Patching SG, Jabbar A, Akbar A, Mustafa MZ. Predominance of Central Asian strain (ST 26) in Mycobacterium tuberculosis isolates from Balochistan by spoligotyping. J Infect Dev Ctries 2019; 13(7): 619-625.
Ahmad I, Nawaz N, Darwesh NM, ur Rahman S, Mustafa MZ, Khan SB, Patching SG. Overcoming challenges for amplified expression of recombinant proteins using Escherichia coli. Protein Expr Purif 2018; 144: 12-18.
Saidijam M, Karimi Dermani F, Sohrabi S, Patching SG. Efflux proteins at the blood-brain barrier: review and bioinformatics analysis. Xenobiotica 2018; 48(5): 506-532.
Patching SG. Recent developments in Nucleobase Cation Symporter-1 (NCS1) family transport proteins from bacteria, archaea, fungi and plants. J Biosci 2018; 43(4): 797-815.
Samad A, Abbas F, Ahmad Z, Tanveer Z, Ahmad I, Patching SG, Nawaz N, Asmat MT, Raziq A, Asadullah, Naeem M, Akhtar MA, Pokryshko O, Mustafa MZ. Multiplex polymerase chain reaction detection of Shiga toxin genes and antibiotic sensitivity of E. coli O157:H7 isolated from beef meat in Quetta, Pakistan. J Food Safety 2018; 38(6): e12540.
Saidijam M, Afshar S, Ahmad I, Patching SG. Nucleoside transporters in PET imaging of proliferating cancer cells using 3'-deoxy-3'-[18F]fluoro-L-thymidine. Journal of Diagnostic Imaging in Therapy 2018; 5(1): 1-13.
Ahmad I, Nawaz N, Ur Rahman S, Sajid A, Khan FA, Khan SB, Mustafa MZ, Patching SG. Multifaceted roles of efflux proteins in prokaryotes. AJBLS 2018; 7(1): 1-7.
Saidijam M, Azizpour S, Patching SG. Comprehensive analysis of the numbers, lengths and amino acid compositions of transmembrane helices in prokaryotic, eukaryotic and viral integral membrane proteins of high-resolution structure. J Biomol Struct Dyn 2018; 36(2): 443-464.
Rahman M, Ismat F, Jiao L, Baldwin JM, Sharples DJ, Baldwin SA, Patching SG. Characterisation of the DAACS family Escherichia coli glutamate/aspartate-proton symporter GltP using computational, chemical, biochemical and biophysical methods. J Membr Biol 2017; 250(2): 145-162.
Patching SG. Glucose transporters at the blood-brain barrier: Function, regulation and gateways for drug delivery. Mol Neurobiol 2017; 54(2): 1046-1077.
Phillips-Jones MK, Channell G, Kelsall CJ, Hughes CS, Ashcroft AE, Patching SG, Dinu V, Gillis RB, Adams GG, Harding SE. Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin. Sci Rep 2017; 7: 46180.
Patching SG. Synthesis, NMR analysis and applications of isotope-labelled hydantoins. Journal of Diagnostic Imaging in Therapy 2017; 4(1): 3-26.
Saidijam M, Azizpour S, Patching SG. Amino acid composition analysis of human secondary transport proteins and implications for reliable membrane topology prediction. J Biomol Struct Dyn 2017; 35(5): 929-949.
Patching SG. NMR-active nuclei for biological and biomedical applications. Journal of Diagnostic Imaging in Therapy 2016; 3(1): 7-48.
Hussain R, Harding SE, Hughes CS, Ma P, Patching SG, Edara S, Siligardi G, Henderson PJ, Phillips-Jones MK. Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions. Biochem Soc Trans 2016; 44(3): 810-823.
Ma P†, Patching SG†, Ivanova E, Baldwin JM, Sharples D, Baldwin SA, Henderson PJ. Allantoin transport protein, PucI, from Bacillus subtilis: evolutionary relationships, amplified expression, activity and specificity. Microbiology 2016; 162(5): 823-836. †Equal contribution by these authors.
Patching SG. Solid-state NMR structures of integral membrane proteins. Mol Membr Biol 2015; 32(5-8): 156-178.
Hiruma-Shimizu K, Shimizu H, Thompson GS, Kalverda AP, Patching SG. Deuterated detergents for structural and functional studies of membrane proteins: Properties, chemical synthesis and applications. Mol Membr Biol 2015; 32(5-8): 139-155.
Patching SG. Roles of facilitative glucose transporter GLUT1 in [18F]FDG positron emission tomography (PET) imaging of human diseases. Journal of Diagnostic Imaging in Therapy 2015; 2(1): 30-102.
Saidijam M, Patching SG. Amino acid composition analysis of secondary transport proteins from Escherichia coli with relation to functional classification, ligand specificity and structure. J Biomol Struct Dyn 2015; 33(10): 2205-2220.
Whittaker CA, Patching SG, Esmann M, Middleton DA. Ligand orientation in a membrane-embedded receptor site revealed by solid-state NMR with paramagnetic relaxation enhancement. Org Biomol Chem 2015; 13(9): 2664-2668.
Jackson SM, Patching SG, Hassan KA, Sharples DJ, Paulsen IT, Hussain R, Siligardi G, Henderson PJF. Tackling bacterial resistance to antiseptics. Diamond Light Source Annual Report, 2014.
Hiruma-Shimizu K, Kalverda AP, Henderson PJ, Homans SW, Patching SG. Synthesis of uniformly deuterated n-dodecyl-β-D-maltoside (d39-DDM) for solubilization of membrane proteins in TROSY NMR experiments. J Labelled Comp Radiopharm 2014; 57(14): 737-743.
Simmons KJ†, Jackson SM†, Brueckner F†, Patching SG†, Beckstein O, Ivanova E, Geng T, Weyand S, Drew D, Lanigan J, Sharples DJ, Sansom MS, Iwata S, Fishwick CW, Johnson AP, Cameron AD, Henderson PJ. Molecular mechanism of ligand recognition by membrane transport protein, Mhp1. EMBO J 2014; 33(16): 1831-1844. †Equal contribution by these authors.
Kalverda AP, Gowdy J, Thompson GS, Homans SW, Henderson PJ, Patching SG. TROSY NMR with a 52 kDa sugar transport protein and the binding of a small-molecule inhibitor. Mol Membr Biol 2014; 31(4): 131-140.
Patching SG. Surface plasmon resonance spectroscopy for characterisation of membrane protein-ligand interactions and its potential for drug discovery. Biochim Biophys Acta – Biomembranes 2014; 1838(1 PtA): 43-55.
Siligardi G, Hussain R, Patching SG, Phillips-Jones MK. Ligand- and drug-binding studies of membrane proteins revealed through circular dichroism spectroscopy. Biochim Biophys Acta – Biomembranes 2014; 1838(1 PtA): 34-42.
Hassan KA, Jackson SM, Penesyan A, Patching SG, Tetu SG, Eijkelkamp BA, Brown MH, Henderson PJ, Paulsen IT. Transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins. Proc Natl Acad Sci U S A 2013; 110(50): 20254-20259.
Patching SG, Jackson SM, Polyakova A, Bettaney KE, Sukumar P, Edara S, Phillips-Jones MK, Henderson PJF, Hussain R, Siligardi G. SRCD analysis of perpetual membrane proteins from bacteria. Diamond Light Source Ltd, Circular Dichroism Beamline B23 Roadshow Series: Membrane Protein and Carbohydrate Research Workshop. The Metropole Hotel, Leeds, 2013.
Middleton DA, Patching SG. Solid-state NMR spectroscopy in drug design and discovery In: Andrushko V, Andrushko N, eds. Stereoselective synthesis of drugs and natural products. Chichester, UK: John Wiley & Sons, Ltd; 2013 (Chapter 51).
Jackson SM, Patching SG, Ivanova E, Simmons KJ, Weyand S, Shimamura T, Brueckner F, Suzuki S, Iwata S, Sharples DJ, Baldwin SA, Sansom MPS, Beckstein O, Cameron A, Henderson PJF (2013) Mhp1, the Na+-hydantoin membrane transport protein. In Roberts GCK (Ed) Encyclopedia of Biophysics, Springer, pp 1514-1521.
Patching SG, Henderson PJ, Sharples DJ, Middleton DA. Probing the contacts of a low-affinity substrate with a membrane-embedded transport protein using 1H-13C cross-polarisation magic-angle spinning solid-state NMR. Mol Membr Biol 2013; 30(2): 129-137.
Patching SG, Edara S, Nakayama J, Phillips-Jones MK. Investigation of FsrC ligand binding, the first step in the Fsr quorum signalling pathway in Enterococcus faecalis. 17th International conference on Bacilli and 7th conference on functional genomics of Gram positive microorganisms, 2013.
Phillips-Jones MK, Patching SG, Edara S, Nakayama J, Hussain R, Siligardi G. Applications of synchrotron radiation circular dichroism (SRCD) for studies of ligand and inhibitor interactions with membrane protein targets: investigations of the FsrC quorum membrane sensor kinase. Society for General Microbiology Spring Meeting, 2013.
Bettaney KE†, Sukumar P†, Hussain R, Siligardi G, Henderson PJ, Patching SG. A systematic approach to the amplified expression, functional characterization and purification of inositol transporters from Bacillus subtilis. Mol Membr Biol 2013; 30(1): 3-14. †Equal contribution by these authors.
Phillips-Jones MK†, Patching SG†, Edara S, Nakayama J, Hussain R, Siligardi G. Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptide inhibitor siamycin I revealed through synchrotron radiation circular dichroism. Phys Chem Chem Phys 2013; 15(2): 444-447. †Equal contribution by these authors.
Phillips-Jones MK, Patching SG, Edara S, Nakayama J, Hussain R, Siligardi G. Investigation of the first step of a virulence regulatory pathway in a bacterial hospital-acquired infection agent. Diamond Light Source Annual Report, 2013.
Patching SG, Middleton DA, Kalverda AP, Gowdy J, Baldwin SA, Phillips-Jones MK, Levitt MH, Homans SW, Henderson PJF. NMR methods for structure-function investigation of membrane proteins and their ligands. J Labelled Comp Radiopharm 2012; 55: 132-133.
Patching SG, Jackson S, Simmons KJ, Ma P, Baldwin SA, Johnson AP, Fishwick CWG, Henderson PJF. Isotope chemistry for investigating ligand binding and transport by Mhp1 and its homologues. J Labelled Comp Radiopharm 2012; 55: 132.
Patching SG, Jacobs TM. Crystal structure of L-5-indolylmethylhydantoin. Private communication to the Cambridge Structural Database, deposition numbers CCDC 865285 and 865286, 2012.
Patching SG. Structure-function investigation of membrane proteins. European Drug Initiative on Channels and Transporters (EDICT) General Assembly, Tavira, Portugal, 15-17 April 2012.
Patching SG, Edara S, Ma P, Nakayama J, Hussain R, Siligardi G, Phillips-Jones MK. Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism. Biochim Biophys Acta – Biomembranes 2012; 1818(7): 1595-1602.
Patching SG. NMR structures of polytopic integral membrane proteins. Mol Membr Biol 2011; 28(6): 370-397. Molecular Membrane Biology cover page: August-September 2011; 28(5-6).
Ma P, Nishiguchi K, Yuille HM, Patching SG, Edara S, Davis LM, Henderson PJF, Nakayama J, Phillips-Jones MK. In vitro activation and inhibition of the intact quorum membrane sensor FsrC involved in biofilms and virulence in Enterococcus faecalis V583. 6th International Conference on Gram-positive Microorganisms, 2011.
Patching SG. Efficient syntheses of 13C- and 14C-labelled 5-benzyl and 5-indolylmethyl L-hydantoins. J Labelled Comp Radiopharm 2011; 54(2): 110-114.
Patching SG. Chemical and NMR methods for structure-function analysis of membrane proteins and their ligands. European Drug Initiative on Channels and Transporters (EDICT) General Assembly, Madrid, Spain, 15-17 April 2011.
Weyand S, Ma P, Saidijam M, Baldwin J, Beckstein O, Jackson S, Suzuki S, Patching SG, Shimamura T, Sansom MSP, Iwata S, Cameron AD, Baldwin SA, Henderson PJF. The Nucleobase‐Cation‐Symport‐1 family of membrane transport proteins. Handbook of Metalloproteins, 2010.
Miller K, Dunsmore CJ, Leeds JA, Patching SG, Sachdeva M, Blake KL, Stubbings WJ, Simmons KJ, Henderson PJ, De Los Angeles J, Fishwick CW, Chopra I. Benzothioxalone derivatives as novel inhibitors of UDP-N-acetylglucosamine enolpyruvyl transferases (MurA and MurZ). J Antimicrob Chemother 2010; 65(12): 2566-2573.
Henderson PJF, Suzuki S, Ma P, Saidijam M, Bettaney K, Szakonyi G, Rutherford NG, Patching SG, Hope RJ, Roach PCJ, Shimamura T, Yajima S, Carpenter EP, Weyand S, Cameron A, Iwata S. Structural genomics of bacterial membrane transport proteins. Acta Crystallographica Section A -Foundations of Crystallography 2009; 65(a1): s28.
Patching SG. Synthesis of highly pure 14C-labelled DL-allantoin and 13C NMR analysis of labelling integrity. J Labelled Comp Radiopharm 2009; 52(9): 401-404.
Patching SG, Edwards R, Middleton DA. Structural analysis of uniformly 13C-labelled solids from selective angle measurements at rotational resonance. J Magn Reson 2009; 199(2): 242-246.
Rahman M, Patching SG, Ismat F, Henderson PJ, Herbert RB, Baldwin SA, McPherson MJ. Probing metal ion substrate-binding to the E. coli ZitB exporter in native membranes by solid state NMR. Mol Membr Biol 2008; 25(8): 683-690.
Weyand S, Shimamura T, Yajima S, Suzuki S, Mirza O, Krusong K, Carpenter EP, Rutherford NG, Hadden JM, O'Reilly J, Ma P, Saidijam M, Patching SG, Hope RJ, Norbertczak HT, Roach PC, Iwata S, Henderson PJ, Cameron AD. Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter. Science 2008; 322(5902): 709-713.
Patching SG, Psakis G, Baldwin SA, Baldwin J, Henderson PJ, Middleton DA. Relative substrate affinities of wild-type and mutant forms of the Escherichia coli sugar transporter GalP determined by solid-state NMR. Mol Membr Biol 2008; 25(6-7): 474-484. Molecular Membrane Biology cover page: September-October 2008; 25(6-7).
Dunsmore CJ, Miller K, Blake KL, Patching SG, Henderson PJ, Garnett JA, Stubbings WJ, Phillips SE, Palestrant DJ, Angeles Jde L, Leeds JA, Chopra I, Fishwick CW. 2-Aminotetralones: novel inhibitors of MurA and MurZ. Bioorg Med Chem Lett 2008; 18(5): 1730-1734.
Patching SG, Henderson PJF. The production of membrane proteins for NMR studies. Collaborative Computational Project for NMR (CCPN) – 8th Conference, Newton Rigg, Penrith, 11-13 August 2008.
Middleton DA, Baldwin SA, Herbert RB, Henderson PJF, Patching SG. Exploiting isotope labelling for solid-state NMR studies of drugs and their targets. J Labelled Comp Radiopharm 2008; 51: 249.
Roach PCJ, O'Reilly J, Norbertczak HT, Hope RJ, Venter H, Patching SG, Jamshad M, Peter G. Stockley PG, Baldwin SA, Herbert RB, Rutherford NG, Bill RM, Henderson PJF. Equipping a research scale fermentation laboratory for production of membrane proteins In: McNeil B, Harvey LM, eds. Practical fermentation technology. Chichester, UK: John Wiley & Sons, Ltd; 2008 (Chapter 3): 37-67.
Patching SG, Henderson PJ, Herbert RB, Middleton DA. Solid-state NMR spectroscopy detects interactions between tryptophan residues of the E. coli sugar transporter GalP and the alpha-anomer of the D-glucose substrate. J Am Chem Soc 2008; 130(4): 1236-1244.
Hadden DA, Loewen S, Ingram JC, Patching SG, Yamashita M, Conroy MJ, Middleton DA, Litherland GJ, Herbert RB, Gallagher MP, Bullough PA, Henderson PJF, Cass CE, Young D, Baldwin SA. Using the bacterial protein NupC as a model for human nucleoside drug transporters. The 4th ISGO International Conference on Structural Genomics. 2006.
Clough J, Saidijam M, Bettaney KE, Szakonyi G, Patching SG, Mueller J, Shibayama K, Bacon M, Barksby E, Groves M, Herbert RB, Phillips-Jones M, Ward A, Gunn-Moore F, O'Reilly J, Rutherford NG, Bill R, Henderson PJF. Prokaryotic membrane transport proteins: amplified expression and purification In Lundstrom KH, ed. Structural genomics on membrane proteins. Taylor and Francis; 2006: 21-42.
Patching SG, Baldwin SA, Baldwin AD, Young JD, Gallagher MP, Henderson PJ, Herbert RB. The nucleoside transport proteins, NupC and NupG, from Escherichia coli: specific structural motifs necessary for the binding of ligands. Org Biomol Chem 2005; 3(3): 462-470.
Saidijam M, Bettaney KE, Szakonyi G, Psakis G, Shibayama K, Suzuki S, Clough JL, Blessie V, Abu-Bakr A, Baumberg S, Meuller J, Hoyle CK, Palmer SL, Butaye P, Walravens K, Patching SG, O'reilly J, Rutherford NG, Bill RM, Roper DI, Phillips-Jones MK, Henderson PJ. Active membrane transport and receptor proteins from bacteria. Biochem Soc Trans 2005; 33(Pt 4): 867-872.
Xie H, Patching SG, Gallagher MP, Litherland GJ, Brough AR, Venter H, Yao SY, Ng AM, Young JD, Herbert RB, Henderson PJ, Baldwin SA. Purification and properties of the Escherichia coli nucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family. Mol Membr Biol 2004; 21(5): 323-336.
Patching SG, Brough AR, Herbert RB, Rajakarier JA, Henderson PJ, Middleton DA. Substrate affinities for membrane transport proteins determined by 13C cross-polarization magic-angle spinning nuclear magnetic resonance spectroscopy. J Am Chem Soc 2004; 126(10): 3072-3080.
Patching SG, Herbert RB, O'Reilly J, Brough AR, Henderson PJ. Low 13C-background for NMR-based studies of ligand binding using 13C-depleted glucose as carbon source for microbial growth: 13C-labeled glucose and 13C-forskolin binding to the galactose-H+ symport protein GalP in Escherichia coli. J Am Chem Soc 2004; 126(1): 86-87.
Patching SG, Herbert RB, Henderson PJF, Brough AR, Middleton DA. Stable isotopes and membrane transport proteins: chemical synthesis and biochemical analysis by solid state NMR. J Labelled Comp Radiopharm 2004; 47: 303.
Patching SG, Middleton DA, Henderson PJ, Herbert RB. The economical synthesis of [2'-13C, 1,3-15N2]uridine; preliminary conformational studies by solid state NMR. Org Biomol Chem 2003; 1(12): 2057-2062.
Sims JL, Carreira JA, Carrier DJ, Crabtree SR, Easton L, Hancock SA, Simcox CE (and Patching SG). A new approach to accelerated drug-excipient compatibility testing. Pharm Dev Technol 2003; 8(2): 119-126.
Patching SG. Chemical and solid-state NMR approaches to determine structure-activity relationships for substrates bound to the nucleoside transport proteins, NupC and NupG, of Escherichia coli. PhD thesis, University of Leeds, 2002. PhD supervisor Dr Richard B. Herbert, external examiner Prof. Tom Simpson B.Sc., D.Sc., Ph.D., F.R.S., F.R.S.C.
Patching SG, Brough AR, Henderson PJF, Baldwin SA, Spooner PJR, Watts A, Gallagher MP, Litherland GJ, Herbert RB. The direct observation by solid state NMR of nucleosides specifically bound to the Escherichia coli membrane transport protein NupC. International workshop “The Future of Solid State NMR in Biology” connected with an official opening of the first European NMR Centre, Leiden, The Netherlands, 2000.
Patching SG, Henderson PJF, Spooner PJR, Watts A, Baldwin SA, Herbert RB. A solid-state NMR approach to the structure-activity relationship of the nucleoside transport protein NupC of Escherichia coli. Biochem Soc Trans 2000; 28: A89.
Patching SG, Gardiner PH. Recent developments in selenium metabolism and chemical speciation: a review. J Trace Elem Med Biol 1999; 13(4): 193-214.
Patching SG. The successful “Design and synthesis of a novel water-soluble functionalised blue dye for protein visualisation in electrophoresis”. BSc(Hons) Biomedical Chemistry - Final year laboratory project thesis, 1998.
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Membrane protein production and studies of structure-function relationships of membrane proteins in microbes, plants, humans and other organisms and in antimicrobial resistance, infectious and foodborne diseases investigated using chemical, biochemical, biophysical, molecular imaging and computational techniques - including nuclear magnetic resonance (NMR) spectroscopy - and the development of methods to achieve this.
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Publications
Ali M, Ali R, Khan IA, Ahmad I, Nawaz N, Patching SG. The Escherichia coli zinc exporter ZitB of the Cation Diffusion Facilitator (CDF) protein family: Properties, cloning, amplified expression, purification. viXra Preprints 2025: 2504.0050. https://vixra.org/abs/2504.0050
Patching SG. Ultra-High Field Nuclear Magnetic Resonance (NMR) Spectroscopy: Applications at 1 GHz and Beyond. International Journal of Current Science Research and Review 2025; 8(1): 336-351. DOI: https://doi.org/10.47191/ijcsrr/V8-i1-3
Nawaz N, Ahmad I, Patching SG, Hussain I. Tribulus terrestris fruit’s potential for synthesizing gold nanoparticles with remarkable biological prospects. Journal of Biological Research 2025. DOI: 10.4081/jbr.2025.12674
Ahmad I, Nawaz N, Afshar S, Saidijam M, Patching, SG. (2024). Roles of Nucleoside Transporters and the Nucleoside Analogue Radiotracer 3’-Deoxy-3’-[18F]Fluoro-L-Thymidine ([18F]FLT) in PET Imaging of Cancer Cells. In: Oluwagbemiga OF (Ed.) Medicine and Medical Research: New Perspectives Vol. 4, 103-137.
Patching SG. Structure and substrate recognition of the Escherichia coli transport protein NupG from the Nucleoside:H+ Symporter (NHS) family. International Journal of Current Science Research and Review 2024; 7(6): 3863-3868.
Ahmad I, Ali M, Ali R, Nawaz N, Patching SG. Multidrug Efflux Protein Families in Bacteria: ABC, RND, MFS, SMR, MATE, PACE, AbgT (An Update). viXra Preprint 2024. https://vixra.org/abs/2406.0087
Patching SG. Ultra-High Field Nuclear Magnetic Resonance (NMR) Spectroscopy: Applications at 1 GHz and Beyond. OSF Preprints 2024. DOI: 10.31219/osf.io/gte42
Patching SG. Structure and substrate recognition of the Escherichia coli transport protein NupG from the Nucleoside:H+ Symporter (NHS) family. OSF Preprints 2024. DOI: 10.31219/osf.io/ht9dg
Nawaz N, Patching SG. Polarising agents and spin tags used in DNP-ssNMR studies on biological samples: An update. Innovations in Biological Science (Vol. 2) 2024, pp. 123-164. DOI: 10.9734/bpi/ibs/v2/8303E
Nawaz N, Ali R, Ali M, Manfield IW, Taj MK, Mustafa MZ, Patching SG. Microscale Thermophoresis Analysis of Membrane Proteins. Chemical Science International Journal 2024; 33(2): 25-45. DOI: 10.9734/CSJI/2024/v33i2887.
Nawaz N, Patching SG. Polarising Agents and Spin Tags for Dynamic Nuclear Polarisation (DNP)-Enhanced Solid-State Nuclear Magnetic Resonance (ssNMR) Analysis of Biological Samples. Current Journal of Applied Science and Technology, 2023; 42(28): 11-38.
Nawaz N, Ali R, Ali M, Manfield IW, Taj MK, Mustafa MZ, Patching SG. Microscale Thermophoresis Analysis of Membrane Proteins. OSF Preprints 2024. DOI: 10.31219/osf.io/pe7ut
Nawaz N, Patching SG. Polarising Agents and Spin Tags for Dynamic Nuclear Polarisation (DNP)-Enhanced Solid-State Nuclear Magnetic Resonance (ssNMR) Analysis of Biological Samples. OSF Preprints 2023. DOI: 10.31219/osf.io/pyjuk
Sun L, Patching SG. Elevator mechanism of alternating access in the Escherichia coli concentrative nucleoside transporter NupC. Int J Adv Multidisc Res Stud 2023; 3(2): 888-910.
Patching SG. Structural and functional mechanisms of membrane proteins: Implications in health and disease (Editorial). Int J Mol Sci 2023.
Patching SG. Bacterial efflux proteins (Editorial). Int J Mol Sci 2023.
Ahmad I, Hassan KA, Henderson PJF, Patching SG. Cloning, amplified expression, functional characterisation and purification of a Pseudomonas putida NCS1 family transport protein. Int J Adv Multidiscip Res 2022; 9(12): 127-156. DOI: 10.22192/ijamr.2022.09.12.01
Basnet S, Marahatha R, Shrestha A, Bhattarai S, Katuwal S, Sharma KR, Marasini BP, Dahal SR, Basnyat RC, Patching SG, Parajuli N. In vitro and in silico studies for the identification of potent metabolites of some high-altitude medicinal plants from Nepal inhibiting SARS-CoV-2 spike protein. Molecules 2022; 27(24): 8957. DOI: 10.3390/molecules27248957
Patching SG. Spermidine binding to the Acetinobacter baumannii efflux protein AceI observed by near-UV synchrotron radiation circular dichroism spectroscopy. Radiation 2022; 2(2): 228-233. DOI: 10.3390/radiation2020016
Ahmad I, Ali M, Ali R, Nawaz N, Patching SG. Structure-based virtual screening and molecular docking of drugs against the SARS-CoV-2 spike protein-ACE2 receptor complex. Pak J Pharm Sci 2022, 35(6): 1531-1538. DOI: 10.36721/PJPS.2022.35.6.REG.1531-1538.1
Nadar S, Khan T, Patching SG, Omri A. Development of antibiofilm therapeutics strategies to overcome antimicrobial drug resistance. Microorganisms 2022; 10(2): 303. DOI: 10.3390/microorganisms10020303
Zia-Ud-Din, Aftab MN, Kakar NH, Mustafa MZ, Zafar A, Iqbal I, Ahmad I, Patching SG, Khan MA, Asmat TM, Shafee M, Rafeeq M. Comparative effects of Bacillus probiotic and antibiotic on the growth performance of indigenous Aseel chickens. Int J Agric Biol Sci 2022; 4: 37-45.
Ahmad I, Lee Y, Nawaz N, Elahi R, Khan IA, Mustafa MZ, Patching SG. Cloning, amplified expression and bioinformatics analysis of a putative Nucleobase Cation Symporter-1 (NCS-1) protein from Rhodococcus erythropolis. BioScientific Review 2021; 3(4). DOI: 10.32350//BSR.0304.05
Tayyeb M, Rashid N, Kakar NH, Taj MK, Abbasi BHA, Ahmad I, Patching SG, Kakar H, Mustafa MZ. Microbial and nutritional evaluation of fresh and wastewater cultivated cabbage in Quetta, Pakistan. Int J Environ Agric Res 2021; 7(5): 15-19.
Ahmad I, Khattak S, Ali R, Nawaz N, Ullah K, Khan SB, Ali M, Patching SG, Mustafa MZ. Prevalence and molecular characterization of multidrug-resistant Escherichia coli O157: H7 from dairy milk in the Peshawar region of Pakistan. J Food Safety 2021; 41(6): e12941. DOI: 10.1111/jfs.12941
Nawaz N, Ahmad I, Patching SG, Hussain I. Green synthesis of gold nanoparticles using Tribulus terrestris extract and antibacterial activity against Gram-negative bacteria. Biomed Lett 2021; 7(2): 80-89. DOI: 10.47262/BL/7.2.20210617
Patching SG. Bacterial multidrug efflux proteins in antimicrobial resistance (Editorial). Antibiotics 2021.
Ahmad I, Hassan KA, Henderson PJF, Patching SG. Cloning, amplified expression, functional characterisation and purification of a Pseudomonas putida NCS1 family transport protein. Preprint 2021.
Ahmad I, Hassan KA, Sharples DJ, Paulsen IT, Patching SG, Henderson PJF. Expression screening and purification of PACE family multidrug efflux proteins. Preprint 2021.
Rabail R, Saleem J, Tanveer Z, Patching SG, Khalid AR, Sultan MT, Manzoor MF, Karrar E, Inam-Ur-Raheem M, Shabbir MA, Aadil RM. Nutritional and lifestyle changes required for minimizing the recovery period in home quarantined COVID‐19 patients of Punjab, Pakistan. Food Sci Nutr 2021; 9(9): 5036-5039.
Patching SG, Martins M, Murakami S, Yan A, Yishino K, Law CJ, Nandanwar HS, Bay DC. Structure, function, and regulation of bacterial efflux proteins in metal homeostasis and antimicrobial resistance (Editorial). Front Mol Biosci 2021.
Ahmad I, Nawaz N, Dermani FK, Kohlan AK, Saidijam M, Patching SG. Bacterial multidrug efflux proteins: A major mechanism of antimicrobial resistance. Curr Drug Targets 2020 [Epub ahead of print] DOI: 10.2174/1389450119666180426103300.
Sun L, Xie H, Ingram JC, Hope RJ, Baldwin SA, Patching SG. Characterization of the Escherichia coli concentrative nucleoside transporter NupC using computational, biochemical and biophysical methods. Biochemistry 2020 [Epub ahead of print] DOI: 10.1021/acs.biochem.7b00172.
Tanveer Z, Ahmad I, Javed MS, Malik SA, Kakr NH, Ahmad M, Patching SG, Naeem M, Mustafa MZ. Screening for hepatitis b and c viral infections among pregnant women attending the Bolan Medical Complex Hospital and Sandeman Provincial Civil Hospital Quetta, Pakistan. Professional Med J 2020; 27(7): 1328-1334. DOI: 10.29309/TPMJ/2020.27.07.3129.
Ali R, Jiao Y, Wall PK, Patching SG, Ahmad I, Lutfulla G, dePamphilis CW. In silico identification and structure function analysis of a putative coclaurine N-methyltransferase from Aristolochia fimbriata. Comput Biol Chem 2020; 85: 107201.
Robajac D, Šunderić M, Gligorijević N, Nedić O, Tingey M, Schnell SJ, Li Y, Junod S, Yu W, Yang W, Roy NK, Beckstein O, Naughton F, Lu M, Lu KS-J, Lekshmi M, Wenzel N, Kumar SH, Varela MF, Turner RJ, Ahmad I, Ma P, Nawaz N, Sharples DJ, Henderson PJF, Patching SG. A Closer Look at Membrane Proteins. Independent Publishing Network, UK, 2020, pp. 1-350. ISBN: 978-1-83853-535-3.
Ahmad I, Ma P, Nawaz N, Sharples DJ, Henderson PJF, Patching SG. Cloning, amplified expression, functional characterisation and purification of Vibrio parahaemolyticus NCS1 cytosine transporter VPA1242. Chapter 8. In: Patching SG (Ed) A Closer Look at Membrane Proteins. Independent Publishing Network, UK, 2020, pp. 241-267. ISBN: 978-1-83853-535-3.
Jehan M, Bajwa MA, Tariq MM, Eyduran E, Rafeeq M, Mustafa MZ, Ahmad I, Patching SG, Waheed A, Ahmad J, Khan MA, Ahmad F, Fatih A, Ali I, Ahmed M, Amin S. Unique characteristics, biometric measurements, management and performance parameters of Balochi sheep. Int J Agric Biol Sci 2020; 4(5): 1-6.
Nawaz N, Ahmad I, Darwesh NM, Wahab A, ur Rahman S, Sajid A, Khan FA, Khan SB, Patching SG, Uddin K. Synthesis, characterization and antioxidant activity of Nickel(II) Schiff base complexes derived from 4-(dimethylamino)benzaldehyde. J Chem Soc Pak 2020; 42(2): 238-242.
Samad A, Abbas F, Tanveer Z, Ahmad Z, Ahmad I, Patching SG, Nawaz N, Asmat MT, Raziq A, Asasullah, Sheikh IS, Naeem M, Pokryshko O, Mustafa MZ. Prevalence of Salmonella spp. in chicken meat from Quetta retail outlets and typing through multiplex PCR. Rom Biotech Lett 2019; 24(2): 271-279.
Hassan KA, Naidu V, Edgerton JR, Mettrick KA, Liu Q, Fahmy L, Li L, Jackson SM, Ahmad I, Sharples D, Patching SG, Henderson PJF, Paulsen IT. Short-chain diamines are the physiological substrates of PACE family efflux pumps. Proc Natl Acad Sci U S A 2019; 116(36): 18015-18020.
Shafee M, Abbas F, Tanveer Z, Whitelaw A, Tow LA, Ashraf M, Ahmad I, Patching SG, Jabbar A, Akbar A, Mustafa MZ. Predominance of Central Asian strain (ST 26) in Mycobacterium tuberculosis isolates from Balochistan by spoligotyping. J Infect Dev Ctries 2019; 13(7): 619-625.
Ahmad I, Nawaz N, Darwesh NM, ur Rahman S, Mustafa MZ, Khan SB, Patching SG. Overcoming challenges for amplified expression of recombinant proteins using Escherichia coli. Protein Expr Purif 2018; 144: 12-18.
Saidijam M, Karimi Dermani F, Sohrabi S, Patching SG. Efflux proteins at the blood-brain barrier: review and bioinformatics analysis. Xenobiotica 2018; 48(5): 506-532.
Patching SG. Recent developments in Nucleobase Cation Symporter-1 (NCS1) family transport proteins from bacteria, archaea, fungi and plants. J Biosci 2018; 43(4): 797-815.
Samad A, Abbas F, Ahmad Z, Tanveer Z, Ahmad I, Patching SG, Nawaz N, Asmat MT, Raziq A, Asadullah, Naeem M, Akhtar MA, Pokryshko O, Mustafa MZ. Multiplex polymerase chain reaction detection of Shiga toxin genes and antibiotic sensitivity of E. coli O157:H7 isolated from beef meat in Quetta, Pakistan. J Food Safety 2018; 38(6): e12540.
Saidijam M, Afshar S, Ahmad I, Patching SG. Nucleoside transporters in PET imaging of proliferating cancer cells using 3'-deoxy-3'-[18F]fluoro-L-thymidine. Journal of Diagnostic Imaging in Therapy 2018; 5(1): 1-13.
Ahmad I, Nawaz N, Ur Rahman S, Sajid A, Khan FA, Khan SB, Mustafa MZ, Patching SG. Multifaceted roles of efflux proteins in prokaryotes. AJBLS 2018; 7(1): 1-7.
Saidijam M, Azizpour S, Patching SG. Comprehensive analysis of the numbers, lengths and amino acid compositions of transmembrane helices in prokaryotic, eukaryotic and viral integral membrane proteins of high-resolution structure. J Biomol Struct Dyn 2018; 36(2): 443-464.
Rahman M, Ismat F, Jiao L, Baldwin JM, Sharples DJ, Baldwin SA, Patching SG. Characterisation of the DAACS family Escherichia coli glutamate/aspartate-proton symporter GltP using computational, chemical, biochemical and biophysical methods. J Membr Biol 2017; 250(2): 145-162.
Patching SG. Glucose transporters at the blood-brain barrier: Function, regulation and gateways for drug delivery. Mol Neurobiol 2017; 54(2): 1046-1077.
Phillips-Jones MK, Channell G, Kelsall CJ, Hughes CS, Ashcroft AE, Patching SG, Dinu V, Gillis RB, Adams GG, Harding SE. Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin. Sci Rep 2017; 7: 46180.
Patching SG. Synthesis, NMR analysis and applications of isotope-labelled hydantoins. Journal of Diagnostic Imaging in Therapy 2017; 4(1): 3-26.
Saidijam M, Azizpour S, Patching SG. Amino acid composition analysis of human secondary transport proteins and implications for reliable membrane topology prediction. J Biomol Struct Dyn 2017; 35(5): 929-949.
Patching SG. NMR-active nuclei for biological and biomedical applications. Journal of Diagnostic Imaging in Therapy 2016; 3(1): 7-48.
Hussain R, Harding SE, Hughes CS, Ma P, Patching SG, Edara S, Siligardi G, Henderson PJ, Phillips-Jones MK. Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions. Biochem Soc Trans 2016; 44(3): 810-823.
Ma P†, Patching SG†, Ivanova E, Baldwin JM, Sharples D, Baldwin SA, Henderson PJ. Allantoin transport protein, PucI, from Bacillus subtilis: evolutionary relationships, amplified expression, activity and specificity. Microbiology 2016; 162(5): 823-836. †Equal contribution by these authors.
Patching SG. Solid-state NMR structures of integral membrane proteins. Mol Membr Biol 2015; 32(5-8): 156-178.
Hiruma-Shimizu K, Shimizu H, Thompson GS, Kalverda AP, Patching SG. Deuterated detergents for structural and functional studies of membrane proteins: Properties, chemical synthesis and applications. Mol Membr Biol 2015; 32(5-8): 139-155.
Patching SG. Roles of facilitative glucose transporter GLUT1 in [18F]FDG positron emission tomography (PET) imaging of human diseases. Journal of Diagnostic Imaging in Therapy 2015; 2(1): 30-102.
Saidijam M, Patching SG. Amino acid composition analysis of secondary transport proteins from Escherichia coli with relation to functional classification, ligand specificity and structure. J Biomol Struct Dyn 2015; 33(10): 2205-2220.
Whittaker CA, Patching SG, Esmann M, Middleton DA. Ligand orientation in a membrane-embedded receptor site revealed by solid-state NMR with paramagnetic relaxation enhancement. Org Biomol Chem 2015; 13(9): 2664-2668.
Jackson SM, Patching SG, Hassan KA, Sharples DJ, Paulsen IT, Hussain R, Siligardi G, Henderson PJF. Tackling bacterial resistance to antiseptics. Diamond Light Source Annual Report, 2014.
Hiruma-Shimizu K, Kalverda AP, Henderson PJ, Homans SW, Patching SG. Synthesis of uniformly deuterated n-dodecyl-β-D-maltoside (d39-DDM) for solubilization of membrane proteins in TROSY NMR experiments. J Labelled Comp Radiopharm 2014; 57(14): 737-743.
Simmons KJ†, Jackson SM†, Brueckner F†, Patching SG†, Beckstein O, Ivanova E, Geng T, Weyand S, Drew D, Lanigan J, Sharples DJ, Sansom MS, Iwata S, Fishwick CW, Johnson AP, Cameron AD, Henderson PJ. Molecular mechanism of ligand recognition by membrane transport protein, Mhp1. EMBO J 2014; 33(16): 1831-1844. †Equal contribution by these authors.
Kalverda AP, Gowdy J, Thompson GS, Homans SW, Henderson PJ, Patching SG. TROSY NMR with a 52 kDa sugar transport protein and the binding of a small-molecule inhibitor. Mol Membr Biol 2014; 31(4): 131-140.
Patching SG. Surface plasmon resonance spectroscopy for characterisation of membrane protein-ligand interactions and its potential for drug discovery. Biochim Biophys Acta – Biomembranes 2014; 1838(1 PtA): 43-55.
Siligardi G, Hussain R, Patching SG, Phillips-Jones MK. Ligand- and drug-binding studies of membrane proteins revealed through circular dichroism spectroscopy. Biochim Biophys Acta – Biomembranes 2014; 1838(1 PtA): 34-42.
Hassan KA, Jackson SM, Penesyan A, Patching SG, Tetu SG, Eijkelkamp BA, Brown MH, Henderson PJ, Paulsen IT. Transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins. Proc Natl Acad Sci U S A 2013; 110(50): 20254-20259.
Patching SG, Jackson SM, Polyakova A, Bettaney KE, Sukumar P, Edara S, Phillips-Jones MK, Henderson PJF, Hussain R, Siligardi G. SRCD analysis of perpetual membrane proteins from bacteria. Diamond Light Source Ltd, Circular Dichroism Beamline B23 Roadshow Series: Membrane Protein and Carbohydrate Research Workshop. The Metropole Hotel, Leeds, 2013.
Middleton DA, Patching SG. Solid-state NMR spectroscopy in drug design and discovery In: Andrushko V, Andrushko N, eds. Stereoselective synthesis of drugs and natural products. Chichester, UK: John Wiley & Sons, Ltd; 2013 (Chapter 51).
Jackson SM, Patching SG, Ivanova E, Simmons KJ, Weyand S, Shimamura T, Brueckner F, Suzuki S, Iwata S, Sharples DJ, Baldwin SA, Sansom MPS, Beckstein O, Cameron A, Henderson PJF (2013) Mhp1, the Na+-hydantoin membrane transport protein. In Roberts GCK (Ed) Encyclopedia of Biophysics, Springer, pp 1514-1521.
Patching SG, Henderson PJ, Sharples DJ, Middleton DA. Probing the contacts of a low-affinity substrate with a membrane-embedded transport protein using 1H-13C cross-polarisation magic-angle spinning solid-state NMR. Mol Membr Biol 2013; 30(2): 129-137.
Patching SG, Edara S, Nakayama J, Phillips-Jones MK. Investigation of FsrC ligand binding, the first step in the Fsr quorum signalling pathway in Enterococcus faecalis. 17th International conference on Bacilli and 7th conference on functional genomics of Gram positive microorganisms, 2013.
Phillips-Jones MK, Patching SG, Edara S, Nakayama J, Hussain R, Siligardi G. Applications of synchrotron radiation circular dichroism (SRCD) for studies of ligand and inhibitor interactions with membrane protein targets: investigations of the FsrC quorum membrane sensor kinase. Society for General Microbiology Spring Meeting, 2013.
Bettaney KE†, Sukumar P†, Hussain R, Siligardi G, Henderson PJ, Patching SG. A systematic approach to the amplified expression, functional characterization and purification of inositol transporters from Bacillus subtilis. Mol Membr Biol 2013; 30(1): 3-14. †Equal contribution by these authors.
Phillips-Jones MK†, Patching SG†, Edara S, Nakayama J, Hussain R, Siligardi G. Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptide inhibitor siamycin I revealed through synchrotron radiation circular dichroism. Phys Chem Chem Phys 2013; 15(2): 444-447. †Equal contribution by these authors.
Phillips-Jones MK, Patching SG, Edara S, Nakayama J, Hussain R, Siligardi G. Investigation of the first step of a virulence regulatory pathway in a bacterial hospital-acquired infection agent. Diamond Light Source Annual Report, 2013.
Patching SG, Middleton DA, Kalverda AP, Gowdy J, Baldwin SA, Phillips-Jones MK, Levitt MH, Homans SW, Henderson PJF. NMR methods for structure-function investigation of membrane proteins and their ligands. J Labelled Comp Radiopharm 2012; 55: 132-133.
Patching SG, Jackson S, Simmons KJ, Ma P, Baldwin SA, Johnson AP, Fishwick CWG, Henderson PJF. Isotope chemistry for investigating ligand binding and transport by Mhp1 and its homologues. J Labelled Comp Radiopharm 2012; 55: 132.
Patching SG, Jacobs TM. Crystal structure of L-5-indolylmethylhydantoin. Private communication to the Cambridge Structural Database, deposition numbers CCDC 865285 and 865286, 2012.
Patching SG. Structure-function investigation of membrane proteins. European Drug Initiative on Channels and Transporters (EDICT) General Assembly, Tavira, Portugal, 15-17 April 2012.
Patching SG, Edara S, Ma P, Nakayama J, Hussain R, Siligardi G, Phillips-Jones MK. Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism. Biochim Biophys Acta – Biomembranes 2012; 1818(7): 1595-1602.
Patching SG. NMR structures of polytopic integral membrane proteins. Mol Membr Biol 2011; 28(6): 370-397. Molecular Membrane Biology cover page: August-September 2011; 28(5-6).
Ma P, Nishiguchi K, Yuille HM, Patching SG, Edara S, Davis LM, Henderson PJF, Nakayama J, Phillips-Jones MK. In vitro activation and inhibition of the intact quorum membrane sensor FsrC involved in biofilms and virulence in Enterococcus faecalis V583. 6th International Conference on Gram-positive Microorganisms, 2011.
Patching SG. Efficient syntheses of 13C- and 14C-labelled 5-benzyl and 5-indolylmethyl L-hydantoins. J Labelled Comp Radiopharm 2011; 54(2): 110-114.
Patching SG. Chemical and NMR methods for structure-function analysis of membrane proteins and their ligands. European Drug Initiative on Channels and Transporters (EDICT) General Assembly, Madrid, Spain, 15-17 April 2011.
Weyand S, Ma P, Saidijam M, Baldwin J, Beckstein O, Jackson S, Suzuki S, Patching SG, Shimamura T, Sansom MSP, Iwata S, Cameron AD, Baldwin SA, Henderson PJF. The Nucleobase‐Cation‐Symport‐1 family of membrane transport proteins. Handbook of Metalloproteins, 2010.
Miller K, Dunsmore CJ, Leeds JA, Patching SG, Sachdeva M, Blake KL, Stubbings WJ, Simmons KJ, Henderson PJ, De Los Angeles J, Fishwick CW, Chopra I. Benzothioxalone derivatives as novel inhibitors of UDP-N-acetylglucosamine enolpyruvyl transferases (MurA and MurZ). J Antimicrob Chemother 2010; 65(12): 2566-2573.
Henderson PJF, Suzuki S, Ma P, Saidijam M, Bettaney K, Szakonyi G, Rutherford NG, Patching SG, Hope RJ, Roach PCJ, Shimamura T, Yajima S, Carpenter EP, Weyand S, Cameron A, Iwata S. Structural genomics of bacterial membrane transport proteins. Acta Crystallographica Section A -Foundations of Crystallography 2009; 65(a1): s28.
Patching SG. Synthesis of highly pure 14C-labelled DL-allantoin and 13C NMR analysis of labelling integrity. J Labelled Comp Radiopharm 2009; 52(9): 401-404.
Patching SG, Edwards R, Middleton DA. Structural analysis of uniformly 13C-labelled solids from selective angle measurements at rotational resonance. J Magn Reson 2009; 199(2): 242-246.
Rahman M, Patching SG, Ismat F, Henderson PJ, Herbert RB, Baldwin SA, McPherson MJ. Probing metal ion substrate-binding to the E. coli ZitB exporter in native membranes by solid state NMR. Mol Membr Biol 2008; 25(8): 683-690.
Weyand S, Shimamura T, Yajima S, Suzuki S, Mirza O, Krusong K, Carpenter EP, Rutherford NG, Hadden JM, O'Reilly J, Ma P, Saidijam M, Patching SG, Hope RJ, Norbertczak HT, Roach PC, Iwata S, Henderson PJ, Cameron AD. Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter. Science 2008; 322(5902): 709-713.
Patching SG, Psakis G, Baldwin SA, Baldwin J, Henderson PJ, Middleton DA. Relative substrate affinities of wild-type and mutant forms of the Escherichia coli sugar transporter GalP determined by solid-state NMR. Mol Membr Biol 2008; 25(6-7): 474-484. Molecular Membrane Biology cover page: September-October 2008; 25(6-7).
Dunsmore CJ, Miller K, Blake KL, Patching SG, Henderson PJ, Garnett JA, Stubbings WJ, Phillips SE, Palestrant DJ, Angeles Jde L, Leeds JA, Chopra I, Fishwick CW. 2-Aminotetralones: novel inhibitors of MurA and MurZ. Bioorg Med Chem Lett 2008; 18(5): 1730-1734.
Patching SG, Henderson PJF. The production of membrane proteins for NMR studies. Collaborative Computational Project for NMR (CCPN) – 8th Conference, Newton Rigg, Penrith, 11-13 August 2008.
Middleton DA, Baldwin SA, Herbert RB, Henderson PJF, Patching SG. Exploiting isotope labelling for solid-state NMR studies of drugs and their targets. J Labelled Comp Radiopharm 2008; 51: 249.
Roach PCJ, O'Reilly J, Norbertczak HT, Hope RJ, Venter H, Patching SG, Jamshad M, Peter G. Stockley PG, Baldwin SA, Herbert RB, Rutherford NG, Bill RM, Henderson PJF. Equipping a research scale fermentation laboratory for production of membrane proteins In: McNeil B, Harvey LM, eds. Practical fermentation technology. Chichester, UK: John Wiley & Sons, Ltd; 2008 (Chapter 3): 37-67.
Patching SG, Henderson PJ, Herbert RB, Middleton DA. Solid-state NMR spectroscopy detects interactions between tryptophan residues of the E. coli sugar transporter GalP and the alpha-anomer of the D-glucose substrate. J Am Chem Soc 2008; 130(4): 1236-1244.
Hadden DA, Loewen S, Ingram JC, Patching SG, Yamashita M, Conroy MJ, Middleton DA, Litherland GJ, Herbert RB, Gallagher MP, Bullough PA, Henderson PJF, Cass CE, Young D, Baldwin SA. Using the bacterial protein NupC as a model for human nucleoside drug transporters. The 4th ISGO International Conference on Structural Genomics. 2006.
Clough J, Saidijam M, Bettaney KE, Szakonyi G, Patching SG, Mueller J, Shibayama K, Bacon M, Barksby E, Groves M, Herbert RB, Phillips-Jones M, Ward A, Gunn-Moore F, O'Reilly J, Rutherford NG, Bill R, Henderson PJF. Prokaryotic membrane transport proteins: amplified expression and purification In Lundstrom KH, ed. Structural genomics on membrane proteins. Taylor and Francis; 2006: 21-42.
Patching SG, Baldwin SA, Baldwin AD, Young JD, Gallagher MP, Henderson PJ, Herbert RB. The nucleoside transport proteins, NupC and NupG, from Escherichia coli: specific structural motifs necessary for the binding of ligands. Org Biomol Chem 2005; 3(3): 462-470.
Saidijam M, Bettaney KE, Szakonyi G, Psakis G, Shibayama K, Suzuki S, Clough JL, Blessie V, Abu-Bakr A, Baumberg S, Meuller J, Hoyle CK, Palmer SL, Butaye P, Walravens K, Patching SG, O'reilly J, Rutherford NG, Bill RM, Roper DI, Phillips-Jones MK, Henderson PJ. Active membrane transport and receptor proteins from bacteria. Biochem Soc Trans 2005; 33(Pt 4): 867-872.
Xie H, Patching SG, Gallagher MP, Litherland GJ, Brough AR, Venter H, Yao SY, Ng AM, Young JD, Herbert RB, Henderson PJ, Baldwin SA. Purification and properties of the Escherichia coli nucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family. Mol Membr Biol 2004; 21(5): 323-336.
Patching SG, Brough AR, Herbert RB, Rajakarier JA, Henderson PJ, Middleton DA. Substrate affinities for membrane transport proteins determined by 13C cross-polarization magic-angle spinning nuclear magnetic resonance spectroscopy. J Am Chem Soc 2004; 126(10): 3072-3080.
Patching SG, Herbert RB, O'Reilly J, Brough AR, Henderson PJ. Low 13C-background for NMR-based studies of ligand binding using 13C-depleted glucose as carbon source for microbial growth: 13C-labeled glucose and 13C-forskolin binding to the galactose-H+ symport protein GalP in Escherichia coli. J Am Chem Soc 2004; 126(1): 86-87.
Patching SG, Herbert RB, Henderson PJF, Brough AR, Middleton DA. Stable isotopes and membrane transport proteins: chemical synthesis and biochemical analysis by solid state NMR. J Labelled Comp Radiopharm 2004; 47: 303.
Patching SG, Middleton DA, Henderson PJ, Herbert RB. The economical synthesis of [2'-13C, 1,3-15N2]uridine; preliminary conformational studies by solid state NMR. Org Biomol Chem 2003; 1(12): 2057-2062.
Sims JL, Carreira JA, Carrier DJ, Crabtree SR, Easton L, Hancock SA, Simcox CE (and Patching SG). A new approach to accelerated drug-excipient compatibility testing. Pharm Dev Technol 2003; 8(2): 119-126.
Patching SG. Chemical and solid-state NMR approaches to determine structure-activity relationships for substrates bound to the nucleoside transport proteins, NupC and NupG, of Escherichia coli. PhD thesis, University of Leeds, 2002. PhD supervisor Dr Richard B. Herbert, external examiner Prof. Tom Simpson B.Sc., D.Sc., Ph.D., F.R.S., F.R.S.C.
Patching SG, Brough AR, Henderson PJF, Baldwin SA, Spooner PJR, Watts A, Gallagher MP, Litherland GJ, Herbert RB. The direct observation by solid state NMR of nucleosides specifically bound to the Escherichia coli membrane transport protein NupC. International workshop “The Future of Solid State NMR in Biology” connected with an official opening of the first European NMR Centre, Leiden, The Netherlands, 2000.
Patching SG, Henderson PJF, Spooner PJR, Watts A, Baldwin SA, Herbert RB. A solid-state NMR approach to the structure-activity relationship of the nucleoside transport protein NupC of Escherichia coli. Biochem Soc Trans 2000; 28: A89.
Patching SG, Gardiner PH. Recent developments in selenium metabolism and chemical speciation: a review. J Trace Elem Med Biol 1999; 13(4): 193-214.
Patching SG. The successful “Design and synthesis of a novel water-soluble functionalised blue dye for protein visualisation in electrophoresis”. BSc(Hons) Biomedical Chemistry - Final year laboratory project thesis, 1998.
Professor Simon G. Patching
Research Scientist / Director, University of Leeds
School of Biomedical Sciences
University of Leeds